Table 3.
Gene set enrichment analysis (GSEA) functional annotation of differentially expressed genes (DEG) in the three conditions.
| Gene Set |
p-Value in vivo Lung |
p-Value in vitro ALI |
p-Value in vitro Submerged |
|---|---|---|---|
| Common gene sets between the three exposures | |||
| IL6_JAK_STAT3_SIGNALING | 1.25 × 10−7 | 7.42 × 10−2 | 3.82 × 10−2 |
| MYC_TARGETS_V2 | 4.30 × 10−6 | 7.42 × 10−2 | 3.65 × 10−2 |
| Common gene sets between in vivo and ALI exposures | |||
| E2F_TARGETS | 1.04 × 10−14 | 8.40 × 10−7 | – |
| G2M_CHECKPOINT | 7.96 × 10−11 | 7.00 × 10−3 | – |
| MYC_TARGETS_V1 | 1.08 × 10−4 | 7.00 × 10−3 | – |
| EPITHELIAL_MESENCHYMAL_TRANSITION | 9.03 × 10−2 | 3.84 × 10−2 | – |
| Common gene sets between in vivo and in vitro submerged exposures | |||
| UV_RESPONSE_DN | 6.21 × 10−3 | – | 6.73 × 10−2 |
| TNFA_SIGNALING_VIA_NFKB | 9.43 × 10−3 | – | 1.46 × 10−3 |
| Non common gene sets | |||
| ALLOGRAFT_REJECTION | 1.25 × 10−7 | – | – |
| MTORC1_SIGNALING | 5.73 × 10−7 | – | – |
| INFLAMMATORY_RESPONSE | 1.48 × 10−6 | – | – |
| MYOGENESIS | 4.30 × 10−6 | – | – |
| INTERFERON_GAMMA_RESPONSE | 8.61 × 10−4 | – | – |
| COMPLEMENT | 5.05 × 10−3 | – | – |
| ANGIOGENESIS | 5.82 × 10−3 | – | – |
| IL2_STAT5_SIGNALING | 2.32 × 10−2 | – | – |
| CHOLESTEROL_HOMEOSTASIS | 3.89 × 10−2 | – | – |
| APICAL_JUNCTION | 4.49 × 10−2 | – | – |
| GLYCOLYSIS | 6.27 × 10−2 | – | – |
| HEDGEHOG_SIGNALING | 6.27 × 10−2 | – | – |
| WNT_BETA_CATENIN_SIGNALING | 8.13 × 10−2 | – | – |
| COAGULATION | 8.89 × 10−2 | – | – |
| TGF_BETA_SIGNALING | 9.03 × 10−2 | – | – |
| OXIDATIVE_PHOSPHORYLATION | 9.03 × 10−2 | – | – |
| UNFOLDED_PROTEIN_RESPONSE | 9.55 × 10−2 | – | – |
| UV_RESPONSE_UP | – | 7.42 × 10−2 | – |
| MITOTIC_SPINDLE | – | – | 9.38 × 10−3 |
| DNA_REPAIR | – | – | 3.65 × 10−2 |
| PROTEIN_SECRETION | – | – | 4.11 × 10−2 |