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. Author manuscript; available in PMC: 2020 Aug 4.
Published in final edited form as: Arch Pathol Lab Med. 2019 Mar 20;143(9):1069–1075. doi: 10.5858/arpa.2018-0466-OA

Figure 3.

Figure 3.

Open-top light-sheet microscopy (OTLS), hematoxylin and eosin (H&E), and immunohistochemistry (IHC) images of a region of prostate carcinoma. H&E and IHC slides were scanned at 20X magnification and the images were cropped to simulate a 5X field of view. A, an OTLS image showing benign prostatic glands (arrow) adjacent to well-formed Gleason pattern 3 glands (arrowhead points to one example). B, an H&E stained slide from the same region as the OTLS image, showing benign prostatic glands (arrow) adjacent to well-formed Gleason pattern 3 glands (arrowhead points to one example). C, a cytokeratin 5 (CK5) immunostain highlights basal cells. The benign glands have a basal cell layer and the carcinoma glands lack a basal cell layer. D, a cytokeratin 8 (CK8) immunostain highlights luminal epithelial cells in both benign and carcinoma glands.