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Open-top light-sheet microscopy (OTLS) images of a carcinoma gland that changes morphology and grade with depth. Each sequential image shows an optical section 40 microns deeper than the previous image. A, three separate well-formed Gleason pattern 3 glands. B and C, a single well-formed Gleason pattern 3 gland. D, a single cribriform Gleason pattern 4 gland.
