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. 2020 Aug 4;15(8):e0235930. doi: 10.1371/journal.pone.0235930

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© 2020 Werckenthin et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — A-C. Injections of Rm´per dsRNA abolished circadian rhythmicity of running wheel locomotor activity in some Madeira cockroaches (n = 4 of 12). (A) Double-plotted running wheel activity of a Madeira cockroach in constant darkness 7 days before, and 29 days after the injection of Rm´per dsRNA (star). At day 36 of the locomotor activity recording the cockroach was sacrificed (cross) and qPCR was used to confirm the knockdown. Chi-square periodogram analysis of the number of running wheel turns indicated significant rhythmicity with a period(τ)of 24.33 h 1–7 days before Rm´per dsRNA injection (B) and loss of rhythmicity 2–4 weeks after the injection (C).