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. 2020 Aug 4;15(8):e0235930. doi: 10.1371/journal.pone.0235930

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© 2020 Werckenthin et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 6 — A-C. Injections of Rm´cry2 dsRNA significantly shortened the free-running period(τ)of locomotor activity rhythms (n = 14 of 20; p<0.0001). (A) Double-plotted running wheel activity of a Madeira cockroach in constant darkness revealed significant shortening in the period of its locomotor activity rhythm after injection of Rm´cry2 dsRNA at day 7 (star) of the recording. At day 36 of the locomotor activity recording the cockroach was sacrificed (cross) and qPCR was used to confirm the knockdown. Chi-square periodogram analysis of the activity 1–7 days before dsRNA injection revealed a period of 23.30 h (B), and 2–4 weeks after the injection of 19.83 h (C).