Fig 8.

A-C. Quantitative PCR (qPCR) showed that Rm´per (A; n = 10), Rm´tim1 (B; n = 10), and Rm´cry2 (C; n = 11) dsRNA injection successfully downregulated mRNA levels of the respective gene (open triangles). The dsRNA experiments revealed only a significant interdependence of Rm´cry2 with Rm´per transcript levels, while Rm´tim1 was independent of both (A-C). Animals that were recorded before in the running wheel assays (Figs 1–6) were sacrificed about one month after injection of dsRNA and their mRNA levels were monitored using qPCR. Open circles and triangles indicate relative expression ratios of individual animals with respect to the mean value of gfp dsRNA injected animals (controls). Kruskal-Wallis tests were used to determine significant differences between gfp (n = 8) and Rm’per/Rm´tim1/Rm´cry2 dsRNA injected animals. ns: not significant; *: p<0.05; **: p<0.01; ***: p<0.001.