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. 2020 Mar 17;1:42–56. doi: 10.1016/j.jvssci.2020.03.001

Fig 4.

Fig 4

Female mice express less Krüppel-like factor 2 (KLF2), endothelial nitric oxide synthase (eNOS), and vascular cell adhesion molecule 1 (VCAM-1) during early arteriovenous fistula (AVF) remodeling. A, Bar graphs show messenger RNA (mRNA) expression of KLF2 in the venous limb of the AVF (P = .0195, analysis of variance [ANOVA]; ∗P = .0108 [post hoc] day 3 vs day 21), p-eNOS (P = .0145, ANOVA; ∗P = .00,318 [post hoc] at day 21; n = 4-5), and VCAM-1 (P < .0001, ANOVA; ∗P < .0001 [post hoc] day 3 vs day 21; n = 4). B, Photomicrographs representative of dual immunofluorescence (IF) of von Willebrand factor (vWF, green) and KLF2 (red, first row) or p-eNOS (red, second row) or 4′,6-diamidino-2-phenylindole (DAPI, blue) and VCAM-1 (green, third row) in male vs female AVF, day 3 vs day 21. L, Lumen; scale bar, 25 μm. C, Bar graphs showing quantification of IF in AVF of male vs female mice; vWF KLF2: P = .0010, ANOVA (day 3 vs day 21, ∗P < .0001); vWF p-eNOS: P = .0022, ANOVA (day 3 vs day 21, ∗P = .0425, post-hoc); VCAM-1: P < .0001, ANOVA (day 3 vs day 21, ∗P = .0007, post hoc; n = 4-5). GAPDH, Glyceraldehyde-3-phosphate dehydrogenase.