REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
BV785 anti-human CD3 (OKT3)	Biolegend	Cat# 317330; RRID: AB_2563507
PE-Dazzle594 anti-human CD4 (OKT4)	Biolegend	Cat# 317448; RRID: AB_2565847
APC-Fire750 anti-human CD8 (SK1)	Biolegend	Cat# 344746; RRID: AB_2572095
BV421 anti-human CD19 (HIB19)	Biolegend	Cat# 302234; RRID: AB_11142678
APC anti-human CD56 (B159)	BD Biosciences	Cat# 555518; RRID: AB_398601
BV605 anti-human CD16 (3G8)	Biolegend	Cat# 302040; RRID: AB_2562990
BV711 anti-human CCR2 (K036C2)	Biolegend	Cat# 357232; RRID: AB_2800970
PE anti-human PD-1 (EH12.2H7)	Biolegend	Cat# 329906, RRID: AB_940483
Percp-cy5.5 anti-human CD38 (HB7)	Biolegend	Cat# 356614; RRID: AB_2562183
AF488 anti-human HLA-DR (LN3)	Biolegend	Cat# 327010; RRID: AB_893568
PE-cy7 anti-human TIM-3 (F38-2E2)	Biolegend	Cat# 345014; RRID: AB_2561720
BV785 anti-human CD19 (HIB19)	Biolegend	Cat# 302240; RRID: AB_2563442
BV785 anti-human CD56 (5.1H11)	Biolegend	Cat# 362550; RRID: AB_2566059
Percp-cy5.5 anti-human CD14 (HCD14)	Biolegend	Cat# 325622; RRID: AB_893250
APC-Fire750 anti-human CD11C	Biolegend	Cat# 371510; RRID: AB_2650793
PE-cy7 anti-human CD123 (7G3)	BD Biosciences	Cat# 560826; RRID: AB_10563898
APC anti-human CD11b (1CRF44)	Biolegend	Cat# 301310; RRID: AB_314162
PE-CF594 anti-human CD33 (WM53)	BD Biosciences	Cat# 562492; RRID: AB_2713912
BV421 anti-human CD15 (W6D3)	Biolegend	Cat# 323040; RRID: AB_2566520
PE anti-human CD86 (BU63)	Biolegend	Cat# 374206; RRID: AB_2721633
PB anti-human CD40 (5C3)	Biolegend	Cat# 334320; RRID: AB_10613295
PE-cy7 anti-human CD80 (2D10)	Biolegend	Cat# 305218; RRID: AB_2076148
PE-CF594 anti-human CD83 (HB15e)	BD Biosciences	Cat# 562631; RRID: AB_2737688
Percp-cy5.5 anti-human CD86 (IT2.2)	Biolegend	Cat# 305420; RRID: AB_1575068
APC anti-human HLA-DR (L243)	Biolegend	Cat# 307610; RRID: AB_314688
FITC anti-human HLA-ABC (W6/32)	Biolegend	Cat# 311404; RRID: AB_314873
PE anti-human CCR7 (G043H7)	Biolegend	Cat# 353204; RRID: AB_10913813
BV711 anti-human CD3 (OKT3)	Biolegend	Cat# 317328; RRID: AB_2562907
Percep-cy5.5 anti-human CD4 (OKT4)	Biolegend	Cat# 317428; RRID: AB_1186122
PE-Dazzle594 anti-human CD8 (RPA-T8)	Biolegend	Cat# 301058; RRID: AB_2563570
PE-cy7 anti-human CD45RA (HI100)	Biolegend	Cat# 304126; RRID: AB_10708879
BV605 anti-human IFN-g (B27)	Biolegend	Cat# 506542; RRID: AB_2801102
APC-cy7 anti-human TNF-a (MAB11)	Biolegend	Cat# 502944; RRID: AB_2562870
AF647 anti-human Granzyme-B (GB11)	Biolegend	Cat# 515406; RRID: AB_2566333
BV421 anti-human Perforin (dG9)	Biolegend	Cat# 308122; RRID: AB_2566204
BV421 anti-human CD141 (M80)	Biolegend	Cat# 344114; RRID: AB_2563858
BV605 anti-human CD123 (6H6)	Biolegend	Cat# 306026; RRID: AB_2563826
APC anti-human CCR7 (G043H7)	Biolegend	Cat# 353214; RRID: AB_10917387
FITC anti-human CD303 (201A)	Biolegend	Cat# 354208; RRID: AB_2561364
PE anti-human CD11c (3.9)	Biolegend	Cat# 301606; RRID: AB_314176
APC-cy7 anti-human CD1c (L161)	Biolegend	Cat# 331520; RRID: AB_10644008
AF488 anti-human ki67 (B56)	BD Biosciences	Cat# 561165; RRID: AB_10611866
Purified Anti-CD3 (OKT3)	Biolegend	Cat# 317347; RRID: AB_2571994
Purified Anti-CD28 (CD28.2)	Biolegend	Cat# 302902; RRID: AB_314304
Anti-human CD49d (9F10)	Biolegend	Cat# 304302; RRID: AB_314428
Biological Samples
Blood sample from healthy donor	Hong Kong Red Cross	N/A
Blood sample from COVID-19 patients	Queen Mary Hospital	N/A
Chemicals, Peptides, and Recombinant Proteins
Human BD Fc block	BD Biosciences	Cat# 564220; RRID: AB_2728082
Lymphoprep	STEMCELL	Cat# 07851
Cell Stimulation Cocktail, PMA/Ionomycin	Biolegend	Cat# 423302
Human IL-1 beta protein	PeproTech	Cat# 200-01B/2ug
Human IL-6	Miltenyi Biotec	Cat# 130-095-365
Human TNF-a	Miltenyi Biotec	Cat# 130-094-017
Prostaglandin E2	PeproTech	Cat# SM-3632464-A
SARS-CoV-2 RBD peptide pool	Genscript Biotech	Custom-made
SARS-CoV-2 Nucleocapsid Protein (NP)	ImmunoDiagnostics	Cat# 41A220
SARS-CoV-2 RBD-His Recombinant Protein	Sino Biological	Cat# 40592-V08B
3, 3′,5, 5′-Tetramethylbenzidine Liquid Substrate	Sigma-Aldrich T4444	Cat# T4444
Goat anti-Human IgG Cross-Adsorbed Secondary Antibody, HRP	Invitrogen	Cat# 62-8420
Critical Commercial Assays
Zombie Aqua™ Fixable Viability Kit	Biolegend	Cat# 308110; RRID: AB_493254
Fixation/Permeabilization Solution Kit	BD Biosciences	Cat# 554714
Pan-DC Enrichment Kit, human	Miltenyi Biotec	Cat# 130-100-777
Pan T Cell Isolation Kit, human	Miltenyi Biotec	Cat# 130-096-535
QuantiNova Probe RT-PCR kit	QIAGEN	Cat# 208352
CellTrace™ CFSE Cell Proliferation Kit	Invitrogen	Cat# C34554
LEGENDplex™ Human Th Panel (13-plex)	Biolegend	Cat# 740722
LEGENDplex™ Human Anti-Virus Response Panel (13-plex)	Biolegend	Cat# 740390
Human IFN-γ ELISpot PLUS kit	MABTECH	Cat# 3420-4APT-2
Luciferase Assay System	Promega	Cat# 1501
Experimental Models: Cell Lines
HEK293T-hACE2 cells	In house	N/A
Software and Algorithms
FLOWJO X 10.0.7r2	https://www.flowjo.com/solutions/flowjo/	RRID: SCR_008520
LEGENDplex v8.0	https://www.biolegend.com/en-us/legendplex	N/A
GraphPad Prism 6	https://www.graphpad.com/scientific-software/prism/	RRID: SCR_002798