(
A) A field of view showing microglial dynamics 1 day after kainate status epilepticus (red: beginning of video; green: end of video). (
B–D) Example images of (
B) a stable process, (
C) extending processes, and (
D) retracting processes from the full field of view in (
A). Roman numerals in (
A) correspond to cells in (
B–D). ∆F/F calcium traces from the highlighted process (white arrow) along with the observed start and end of an extension or retraction event (vertical dotted lines). The relative timing of the nearest calcium event to the start of extension/retraction was used to create the dataset in
Figure 5H. Note: stable processes would not have a start or end to motility and are therefore not included in any temporal analysis.