Author response image 4. Longitudinal survey of active microglia in the kainate cohort.

(Left) The survey method was used to observe any clear calcium event within any part of a cell (green outline) or confirm no observable event in the 15-minute recording (red outline). This example image can also be watched as Figure 5—video 1: Spreading Calcium Wave. In recordings from another day, the inactive cell (#2, red outline) did show somatic calcium activity, suggesting the cell did have GCaMP expression when studied longitudinally. (Right) Percentage of microglia exhibiting calcium activity within one 300 x 300 µm region surveyed over six days (performed across N=5 mice).