Figure 6.

Regulation of KIAA1199 expression by miR‐486‐5p. A, The table showed the predicted miR‐486‐5p binding sites at position 895‐901 and 2374‐2385 of KIAA1199 3′‐UTR. B, The mRNA levels of KIAA1199 were lower in miR‐486‐5p‐upregulated cells. C, The mRNA levels of KIAA1199 were higher in miR‐486‐5p‐inhibited cells than control cells. D, and E, NSCLC cells were co‐transfected with wild‐type or mutant recombinant plasmid and negative control or miR‐486‐5p mimics. Relative luciferase activity was detected 48 hours afterward. Each experiment was performed in triplicate. Significant differences: **P < .01, ***P < .001