Fig. 4. Effect of PNS on levels of ROS, GSH, GSSH, and T-GSH in the spleen of PCV2-infected mice. Negative control: the mice were not infected with PCV2 and not treated with PNS and VC. PCV2: the mice were infected with 10⁴TCID₅₀ PCV2; PCV2 + PNS 50–200 mg/kg bw: the mice were treated with PNS at concentration of 50, 100, or 200 mg/kg bw after 10⁴TCID₅₀ PCV2 infection respectively; PCV2 + VC: the mice were treated with VC at concentration of 200 µM after 10⁴TCID₅₀ PCV2 infection. PNS: the mice were treated with 200 mg/kg bw of PNS without PCV2 infection. The values are represented mean ± SD. Bars with different letters are statistically different (p < 0.05).

ROS, reactive oxygen species; PCV2, porcine circovirus type 2; PNS, Panax notoginseng saponins; VC, vitamin C; GSH, glutathione; GSSG, oxidized glutathione; T-GSH, total glutathione; TCID₅₀, median tissue culture infectious dose.