Fig. 2.

Inhibitions of selected flaviviral polymerases by remdesivir triphosphate. A) IC₅₀ values were established for each flaviviral RdRp tested using the fluorescence based alkaline phosphatase-coupled polymerase assay. B) Gel-based polymerase assay - The incorporation of RemTP incorporation was monitored for each flaviviral RdRp tested (substrate: GTP, RemTP, CTP, UTP) and compared with the natural condition (substrate: GTP, ATP, CTP, UTP). Left - polymerization reaction performed with YFV polymerase with four NTP s followed by a reaction performed with RemTP instead of ATP, next is just the template/primer (T/P) followed by control reactions performed with JEV polymerase in the presence of indicated NTPs, followed by comparison of reactions with ATP/RemTP using indicated flaviviral polymerases. 20 bp band corresponds to unreacted primer, the sequence of nucleotides added by the polymerase is shown on the left side of the gel.