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Determination of the isoelectric point of the Orf virus (ORFV). Zeta potential measurements of virus purified by steric exclusion chromatography (SXC) and the corresponding buffer over a pH range from 2 to 13 (A), and more detailed resolution for the apparent transition from positive to negative zeta potential between pH 4 and 5 (B). At a pH of 4.61 the net charge is zero. Error bars represent standard deviations of triplicate measurements.
