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. 2020 May 18;123(3):378–391. doi: 10.1038/s41416-020-0891-x

Fig. 5. Deciphering the molecular mechanisms of PaRi.

Fig. 5

a RNA-seq identified commonly deregulated transcripts in GCT cell lines 16 h after 10 μM palbociclib treatment. In MPAF control cells, 10 of the 23 transcripts were also deregulated (≥fold change 1.5) after palbociclib application (red labelled genes). b STRING-based interaction prediction of the commonly deregulated genes. Genes also deregulated in fibroblasts are labelled in red. c DAVID-based GO analysis of the commonly deregulated genes. d Validation of RNA-seq data by qRT-PCR in GCT cell lines and MPAF fibroblast treated with PaRi (10 μM/25 μM) for 16 h. GAPDH was used as housekeeper. Data are given as fold changes (PaRi treated vs. solvent control). Standard deviation is given above bars. e Validation of AURKB downregulation on protein level by western blotting. GCT cells were treated with PaRi (10 μM/25 μM) for 16 h. β-ACTIN was used as housekeeper. Densitometric evaluation of western blot data is given on the right side.