Table 1.
Antibodies used in this study.
| Antibody | Company | Clone | Order no. | Dilution | Application |
|---|---|---|---|---|---|
| Primary antibodies | |||||
| β-ACTIN | Sigma-Aldrich | AC-15 | A5441 | 1:20000 | Western blot |
| GAPDH | Abcam | 6C5 | ab8245 | 1:30000 | Western blot |
| RB1 | Cell Signalling Technology | D20 | 9319S | 1:500 | Western blot |
| pRB1 | Abcam | ab4784 | 40615 | 1:1000 | Western blot |
| CDK4 | Santa Cruz Biotechnology | DCS31 | sc-56277 | 1:1500 | Western blot |
| CDK4 | Thermo Fisher Scientific | DCS31 | AHZ0202 | 1:40 | Immunohistochemistry |
| CDK6 | Santa Cruz Biotechnology | B-10 | sc-7961 | 1:1000 | Western blot |
| CyclinD1 | Cell Signalling Technology | 92G2 | 2978 | 1:1000 | Western blot |
| CyclinD2 | BD Pharmingen | G132-43 | 554200 | 1:500 | Western blot |
| Cleaved PARP | Cell Signalling Technology | D214 | 9541S | 1:500 | Western blot |
| PARP | Cell Signalling Technology | 46D11 | 46D11 | 1:1000 | Western blot |
| Aurora kinase B | BD Pharmingen | AIM-1 | 611083 | 1:500 | Western blot |
| Ki-67 | Santa Cruz Biotechnology | MIB-1 | sc-101861 | 1:50 | Immunofluorescence |
| Phospho-H3 | Cell Signalling Technology | D7N8E | 53348 | 1:1600 | Flow cytometry |
| Secondary antibodies | |||||
| Polyclonal rabbit anti-mouse HRP | Dako | P026002-2 | 1:1000 | Western blot/IHC | |
| Polyclonal goat anti-rabbit HRP | Dako | P044801-2 | 1:2000 | Western blot/IHC | |
| Goat anti-mouse IgG (H+L) Alexa Fluor 488 | Thermo Fisher Scientific | A11029 | 1:2000 | Immunofluorescence | |
| Goat anti-rabbit IgG (H+L) Alexa Fluor 488 | Thermo Fisher Scientific | A11034 | 1:2000 | Immunofluorescence | |