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. 2020 Jul 29;8:861. doi: 10.3389/fbioe.2020.00861

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Copyright © 2020 Chopra, Bobate, Rahi, Banpurkar, Mazumder and Satpute.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) A 24 h old culture of Pseudomonas aeruginosa RTE4 grown on Luria agar. (B) Phylogenetic tree based on the 16S rRNA gene sequences, showing the relationship between the strain RTE4 and other members of the genus Pseudomonas. The tree was reconstructed by the neighbor-joining method using the Kimura 2-parameter method (rooted with Oblitimonas alkaliphile B4199^T as an outgroup). Bootstrap values (expressed as percentages of 1,000 replications) above 50% are shown at branch points. Bar, 0.005 substitutions per nucleotide position. (C) Protease activity of RTE4 on skim milk agar plate. (D) Cellulase activity of RTE4 on cellulose Congo red agar plate. Clear halo around spot inoculated RTE4 is an indicative of cellulose production by RTE4.