TABLE 1.

Vitrification and thawing protocols.

Solutions	Compositions	Times
Vitrification protocols
SV-1	TCM199 + 10 mg/mL BSA + 0.25 M sucrose + 10% EG + 10% DMSO	4 min
SV-2	TCM199 + 10 mg/mL BSA + 0.25 M sucrose + 20% EG + 20% DMSO	1 min
Warming protocols:
WS-1	TCM199 + 3 mg/mL BSA + 0.5 M sucrose	5 min
WS-2	TCM199 + 3 mg/mL BSA + 0.25 M sucrose	5 min
WS-3	TCM199 + 3 mg/mL BSA	5 min
BM	PBS + 20% FBS	1–12 h

SV-1, vitrification solutions-1; SV-2, vitrification solutions-2; WS, washing solution; BM, base medium; BSA, bovine serum albumin; EG, ethylene glycol; DMSO, dimethyl sulfoxide; FBS, fetal bovine serum.