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. 2020 Aug 4;10:13148. doi: 10.1038/s41598-020-69960-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Structural, mechanical, and cell viability analysis of spheroids: (A) Diameter change over 26 days (n = 8,***p < 0.001 shows significance between ADSC and chondrogenic spheroids, and ###p < 0.001 shows significance between osteogenic and chondrogenic spheroids), (B) surface tension at Day 26 (n = 5, ***p < 0.001), and (C) SEM images of ADSC, chondrogenic and osteogenic spheroids. (D) LIVE/DEAD images (E) and cell viability in ADSC, chondrogenic, and osteogenic spheroids at Day 26.