Table 4.
Results of univariate analysis of PD-L1, PD-1, CD8, CD4 and CD3 in relation to durable clinical benefit (DCB) are shown for biopsies taken before chemotherapy (BCT) and biopsies taken after the last line of chemotherapy (ACT).
| BCT biopsies | ACT biopsies | ||||||
|---|---|---|---|---|---|---|---|
| DCB rates (%) | Univariate OR (95% CI) | p value | DCB rates (%) | Univariate OR (95% CI) | p value | ||
| Tumour PD-L1 expression | 0.88 | Tumour PD-L1 expression | 0.013 | ||||
| <1% (n = 52) | 21 | 1 | <1% (n = 47) | 23 | 1 | ||
| 1–49% (n = 7) | 14 | 0.62 (0.068–5.7) | 1–49% (n = 9) | 22 | 0.94 (0.17–5.2) | ||
| ≥50% (n = 8) | 25 | 1.2 (0.22–7.0) | ≥50% (n = 13) | 69 | 7.4 (1.9–28.6) | ||
| Stromal infiltration of PD-L1+ IC | 0.60 | Stromal infiltration of PD-L1+ IC | 0.22 | ||||
| Low infiltration (n = 39) | 26 | 1 | Low infiltration (n = 45) | 24 | 1 | ||
| Intermediate infiltration (n = 15) | 13 | 0.45 (0.085–2.3) | Intermediate infiltration (n = 14) | 43 | 2.3 (0.66–8.2) | ||
| High infiltration (n = 11) | 18 | 0.64 (0.12–3.5) | High infiltration (n = 8) | 50 | 3.1 (0.66–14.5) | ||
| Stromal infiltration of PD-1+ IC | 0.55 | Stromal infiltration of PD-1+ IC | 0.006 | ||||
| Low infiltration (n = 40) | 25 | 1 | Low infiltration (n = 41) | 17 | 1 | ||
| Intermediate infiltration (n = 17) | 12 | 0.40 (0.078–2.1) | Intermediate infiltration (n = 17) | 41 | 3.4 (0.96–12.0) | ||
| High infiltration (n = 9) | 22 | 0.86 (0.15–4.8) | High infiltration (n = 12) | 67 | 9.7 (2.3–41.4) | ||
| Tumour infiltration of CD8+ IC | 0.52 | Tumour infiltration of CD8+ IC | 0.009 | ||||
| Low infiltration (n = 37) | 22 | 1 | Low infiltration (n = 39) | 18 | 1 | ||
| Intermediate infiltration (n = 11) | 9 | 0.36 (0.040–3.3) | Intermediate infiltration (n = 16) | 31 | 2.1 (0.55–7.9) | ||
| High infiltration (n = 18) | 28 | 1.4 (0.38–5.1) | High infiltration (n = 16) | 63 | 7.6 (2.1–28.0) | ||
| Stromal infiltration of CD8+ IC | 0.74 | Stromal infiltration of CD8+ IC | 0.044 | ||||
| Low infiltration (n = 11) | 18 | 1 | Low infiltration (n = 18) | 17 | 1 | ||
| Intermediate infiltration (n = 28) | 18 | 0.98 (0.16–6.0) | Intermediate infiltration (n = 17) | 18 | 1.1 (0.19–6.2) | ||
| High infiltration (n = 27) | 26 | 1.6 (0.27–9.1) | High infiltration (n = 35) | 46 | 4.2 (1.0–17.2) | ||
| Stromal infiltration of CD3+ IC | 0.20 | Stromal infiltration of CD3+ IC | 0.035 | ||||
| Low infiltration (n = 23) | 9 | 1 | Low infiltration (n = 26) | 19 | 1 | ||
| Intermediate infiltration (n = 26) | 31 | 4.7 (0.88–24.9) | Intermediate infiltration (n = 23) | 26 | 1,5 (0.39–5.7) | ||
| High infiltration (n = 17) | 24 | 3.2 (0.52–20.2) | High infiltration (n = 20) | 55 | 5.1 (1.4–19.1) | ||
| Stromal infiltration of CD4+ IC | 0.31 | Stromal infiltration of CD4+ IC | 0.10 | ||||
| Low infiltration (n = 16) | 19 | 1 | Low infiltration (n = 15) | 20 | 1 | ||
| Intermediate infiltration (n = 19) | 11 | 0.51 (0.074–3.5) | Intermediate infiltration (n = 17) | 18 | 0.86 (0.15–5.1) | ||
| High infiltration (n = 31) | 29 | 1.8 (0.41–7.8) | High infiltration (n = 37) | 43 | 3.0 (0.74–12.6) | ||
| Combined tumour and stromal infiltration of CD8+ IC | 0.64 | Combined tumour and stromal infiltration of CD8+ IC | 0.008 | ||||
| Low infiltration of tumour and stromal CD8+ IC (n = 28) | 21 | 1 | Low infiltration of tumour and stromal CD8+ IC (n = 31) | 19 | 1 | ||
| High infiltration of tumour or stromal CD8+ IC (n = 20) | 15 | 0.65 (0.14–3.0) | High infiltration of tumour or stromal CD8+ IC (n = 11) | 9 | 0.42 (0.044–3.9) | ||
| High infiltration of tumour and stromal CD8+ IC (n = 18) | 28 | 1.4 (0.36–5.6) | High infiltration of tumour and stromal CD8+ IC (n = 28) | 54 | 4.8 (1.5–15–3) | ||
| Combined stromal infiltration of PD-1+ and CD8+ IC | 0.62 | Combined stromal infiltration of PD-1+ and CD8+ IC | 0.001 | ||||
| Low infiltration of stromal PD-1+ and CD8+ IC (n = 31) | 19 | 1 | Low infiltration of stromal PD-1+ and CD8+ IC (n = 30) | 20 | 1 | ||
| High infiltration of stromal PD-1+ or CD8+ IC (n = 17) | 29 | 1.7 (0.44–6.8) | High infiltration of stromal PD-1+ or CD8+ IC (n = 15) | 7 | 0.29 (0.031–2.6) | ||
| High infiltration of stromal PD-1+ and CD8+ IC (n = 18) | 17 | 0.83 (0.18–3.8) | High infiltration of stromal PD-1+ and CD8+ IC (n = 24) | 63 | 6.7 (2.0–22.5) | ||
| Combined stromal infiltration of PD-L1+ and CD8+ IC | 0.44 | Combined stromal infiltration of PD-L1+ and CD8+ IC | 0.043 | ||||
| Low infiltration of stromal PD-L1+ and CD8+ IC (n = 31) | 19 | 1 | Low infiltration of stromal PD-L1+ and CD8+ IC (n = 30) | 17 | 1 | ||
| High infiltration of stromal PD-L1+ or CD8+ IC (n = 15) | 33 | 2.1 (0.52–8.4) | High infiltration of stromal PD-L1+ or CD8+ IC (n = 20) | 35 | 2.7 (0.71–10.2) | ||
| High infiltration of stromal PD-L1+ and CD8+ IC (n = 19) | 16 | 0.78 (0.17–3.6) | High infiltration of stromal PD-L1+ and CD8+ IC (n = 17) | 53 | 5.6 (1.5–21.8) |
Statistically significant p < 0.05 values are in bold