Table 1.

Putative cryptic acceptor and donor splice sites within rtTA. Dinucleotide splice sites (AG or GT) are highlighted in bold and mutated sequences are underlined.

Name	Type	Original sequence	Mutated sequence	Mutation type
215	Acceptor	CCC CTG GAA GGC GAG TCA Pro Leu Glu Gly Glu Ser	CCC CTG GAT GGC GAG TCA Pro Leu Asp Gly Glu Ser	Missense (conservative)
320	Acceptor	CCA ACA GAG AAA CAG TAC Pro Thr Glu Lys Gln Tyr	CCA ACA CAA AAA CAG TAC Pro Thr Gln Lys Gln Tyr	Missense (conservative)
326	Donor	CCA ACA GAG AAA CAG TAC Pro Thr Glu Lys Gln Tyr	CCA ACA GAG AAA CAA TAC Pro Thr Glu Lys Gln Tyr	Silent
367	Acceptor	TGT CAG CAA GGC TTC TCC Cys Gln Gln Gly Phe Ser	TGT CAA CAA GGC TTC TCC Cys Gln Gln Gly Phe Ser	Silent
392	Donor	AAC GCA CTG TAC GCT CTG Asn Ala Leu Tyr Ala Leu	AAC GCA TTA TAC GCT CTG Asn Ala Leu Tyr Ala Leu	Silent
408	Donor	TCC GCC GTG GGC CAC TTT Ser Ala Val Gly His Phe	TCC GCC ATC GGC CAC TTT Ser Ala Ile Gly His Phe	Missense (conservative)
456	Donor	GAG CAT CAA GTA GCA AAA Glu His Gln Val Ala Lys	GAG CAT CAA GTG GCA AAA Glu His Gln Val Ala Lys	Silent
541	Acceptor	GAC CGG CAG GGA GCC GAA Asp Arg Gln Gly Ala Glu	GAC CGG CAA GGA GCC GAA Asp Arg Gln Gly Ala Glu	Silent