Figure 5.

Transduced BMDCs Induce Functional CD8⁺ T Cells

BMDCs were transduced with lentiviral vectors expressing mCD40L or mCD40L with GP_66–77 or transduced with mCD40L lentiviral vector and peptide pulsed. The BMDCs were injected into mice and 5 days later, infected with LCMV. (A) The splenocytes were stained for CD8 and GP_33–41 tetramer binding and analyzed by flow cytometry. The results are plotted as the percentage tetramer⁺ cells of CD8⁺ cells (n = 3–4). (B) Splenocytes from the mice were stained with GP_33–41 tetramer and anti-CD8 antibody and for different markers of CTL function (granzyme B, perforin, CD43, KLRG1, CX3CR1, CD62L, and CD27) and analyzed by flow cytometry. Data represent mean ± SD. Significance determined by Mann-Whitney U tests.