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. 2020 Aug 4;10:13076. doi: 10.1038/s41598-020-69980-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Differentiated ethanol treatment effect on stress-related behaviors between lines with no effect on locomotion. We measured top transitions (a), time in top half of the tank (b), average time spent in top half per trip (c), distance traveled (d), and stationary time (e) for each treatment group. Control groups are represented by unfilled in bars, while ethanol-treated groups are represented by filled bars. HSB and LSB are red and purple, respectively. Data shown are mean ± 1 SEM. Significant line and treatment differences are indicated by an asterisk (p ≤ 0.05), while differences between groups are indicated by different lower-case letters. The number of subjects tested in each group are as follows: 17 HSB control, 17 LSB control, 15 HSB EtOH, 16 LSB EtOH.