Figure 1.

Murine Model of AS Recapitulates Representative Features of AS in Humans

(A) A schematic diagram to illustrate the induction and observation schedule for a murine model of human AS (n = 5 mice in each group). (B) Masson’s trichrome (MT) staining 3, 7, and 14 days after uterine damage to evaluate the degree of the damage. Blue and red colors indicate collagen and cytoplasm, respectively. LE, luminal epithelium; GE, glandular epithelium; S, stroma. Scale bar, 100 μm. (C) Real-time RT-PCR analyses for relative mRNA levels of fibrosis-related factors in uteri with AS. ∗p < 0.05, ∗∗p < 0.01. (D) Western blotting for COL1A1 and TGF-β1 proteins in AS uteri after damage. GAPDH was used as a loading control. (E) Comparison of MT staining and immunofluorescence (IF) staining of COL1A1 in endometrial tissues from normal controls and AS patients (n = 3 patients in each group) and from AS uteri in mice. Scale bar, 100 μm.