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. 2020 May 20;28(8):1818–1832. doi: 10.1016/j.ymthe.2020.05.015

Figure 5.

Figure 5

hPVSCs Contribute to Uterine Regeneration in a Paracrine Fashion

(A) A schematic diagram illustrating the experimental procedures for the intravenous (i.v.) delivery of hPVSCs to mice with AS. AS was induced in one uterine horn while the other horn served as the sham control in the same mouse (n = 4–6 mice in each group). (B) Observation of the antifibrotic functions of hPVSCs delivered i.v. into uteri with AS. Gross histology with H&E staining and IF staining of COL1A1 and immunohistochemical staining of KI-67 in uteri with AS after i.v. delivery of hPVSCs. Green and red colors indicate COL1A1 and nuclei, respectively. Brown indicates KI-67-positive uterine cells. LE, luminal epithelium; GE, glandular epithelium; S, stroma, Scale bar, 100 μm. (C) Prussian blue (PB) staining of iron deposits to track hPVSCs in the uterus after i.v. delivery. Scale bar, 25 μm. (D) Quantitation of PB-positive hPVSCs in the uterus after i.v. delivery. ∗p < 0.05, ∗∗p < 0.01.