Figure 4.

Anti-tumoral Activity at the Cellular Level

(A–D) Cells isolated from the bone marrow, spleen, and blood were evaluated by flow cytometry for the percentage of human CD3⁺ within the total cells (A), CAR⁺ cells in the CD4⁺CD3⁺ (B) and the CD8⁺CD3⁺ fractions (C), and CD19⁺ cells within total cells (D) at 14 and 21 days after vector administration. The gating strategy is represented in Figure S4. Data represents mean ± SEM for all groups (control, n = 6; CD4-LV, n = 7; CD8-LV, n = 6; MIX, n = 7). Statistical significance was determined using a two-way ANOVA. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.