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. 2020 Aug 4;10:13143. doi: 10.1038/s41598-020-69735-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RNA-seq data from lung endothelial cells isolated from DOL-4 mice treated with LPS for 24 h. (A) Heatmap of differentially expressed genes (n = 3); (B) top pathways identified in Ingenuity Pathway Analysis as activated; and (C) validation RT-PCR in primary mouse lung endothelial cells (n = 6). Data presented at mean ± standard deviation, *p < 0.001 between LPS-treated and saline control baseline by one-sample, two-tailed Student’s t test as all data were normally distributed). Graphs created in SPSS 26 for Mac (https://www.ibm.com/analytics/spss-statistics-software) and image assembled in Adobe Illustrator 24.1.0 for Mac (https://www.adobe.com/products/illustrator.html).