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. 2020 Aug 4;10:13143. doi: 10.1038/s41598-020-69735-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Flowchart of gene identification. Describing identification of genes of interest, starting from identification of genes with statistically significant changes in expression at 6 h that were not known to be influenced by LPS, removal of non-novel findings, RT-PCR validation and linkage to genes with statistically significant changes in expression at 24 h that were known to be angiogenic or vasculogenic, had at least two-fold change in expression, and not found to be linked to Fosl1 or LPS in the IPA-KB, validated by RT-PCR resulted in the final list of Prok2, Lrg1, Adam8, and Rnf213. Image created in Adobe Illustrator 24.1.0 for Mac (https://www.adobe.com/products/illustrator.html).