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. 2020 Aug 4;10:13143. doi: 10.1038/s41598-020-69735-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Validation of cell line as endothelial cells. (A) expression of ERG, CDH5, and vWF by immunoflourescence, and uptake of AcLDL; (B) assessment of novel LPS genes after treatment of primary HPMECs (n = 3) and HPMEC line (n = 4) with LPS for 6 h (p values determined by one-sample, two-tailed Student’s t test); and (C) assessment of angiogenesis or vasculogenesis genes after treatment of primary HPMECs (n = 3) and HPMEC line (n = 5) with LPS for 24 h (p values determined by one-sample, two-tailed Student’s t test; all data were normally distributed except 24 h primary HPMEC LRG1 which was assessed with one-sample Wilcoxon signed rank test). Data presented at mean ± standard deviation. *p < 0.05, **p < 0.01, ***p < 0.001 between LPS-treated and saline control baseline. Graphs created in SPSS 26 for Mac (https://www.ibm.com/analytics/spss-statistics-software) and image assembled in Adobe Illustrator 24.1.0 for Mac (https://www.adobe.com/products/illustrator.html).