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. 2020 Aug 4;10:13129. doi: 10.1038/s41598-020-69570-2

Figure 1.

Figure 1

Ce6-PVP is suitable for optical imaging of colorectal cancer cells in vitro. (a, b) MC-38 and Caco-2 cells were either treated with Ce6-PVP [15 µM] for 20 min or left untreated as controls. Cells were washed afterwards with PBS to remove unbound Ce6-PVP. (a) Emission scan of Ce6-PVP fluorescence intensity (excitation wavelength 405 nm) with an optical photometer 4 h after treatment. X-axis: Emission wavelength [nm], Y-axis: fluorescence intensity. Shown is one representative measurement out of three (Caco-2) or two (MC38) iterations of the experiment. (b) Confocal microscope images. Imaging sequence was begun within 1 h after treatment. Images are representative of two independent experiments (Caco-2) or display one experiment (MC38). (c) Murine colon tumor organoids were generated and either treated with Ce6-PVP [15 µM] for 30 min or left untreated as controls. Organoids were washed with PBS to remove unbound Ce6-PVP and imaged afterwards. Confocal microscope images. Imaging sequence was begun within 1 h after treatment. Images are representative of two independent experiments.