Skip to main content
. 2020 May 18;123(3):426–437. doi: 10.1038/s41416-020-0884-9

Fig. 6. miR-622 affects cell motility in breast cancer cell lines through targeting NUAK1.

Fig. 6

a Matrigel invasion assay was performed in MDA-MB-231 cells transfected with miR-622 (left) or with Anti-miR-622 (right) and relative controls. After 24 h, eluted invading cells were quantified at 550 nm O.D. and reported as relative invasion ability (%). b Matrigel invasion assays were used to evaluate the invasion ability of MCF-7 after stable transfection with miR-622 (left) or Anti-miR-622 (right) and their relative controls. c Representative images of MDA-MB-231 transfected cells plated in Matrigel 3D at different time point. d After transient transfection of NUAK1 or Empty vector in MDA-MB-231/miR-Null and in MDA-MB-231/miR-622 cells, wound gaps were inflicted, photographed (left) and measured (right) at 0- and 24-h time points. Data shown are from two independent experiments ± S.D. *p < 0.05; **p < 0.01.