Fig. 4. Effect of LCOs, COs, and fatty acids on C. glabrata.
a White arrows show pseudohyphae of C. glabrata (see also Supplementary Movies 1, 2, and 3). Scale bar is 10 µm. b The number of pseudohyphae observed per well after treatment for 12 h with various LCOs, COs, and fatty acids at 10−8 M. Different letters indicate significant differences and similar letters indicate no difference according to Tukey’s single-step multiple comparison procedure; one-way ANOVA p-value is 9.90 × 10−10. Each treatment was analyzed in four independent wells. c Effect of a range of C16:0 sulfated LCO concentrations on pseudohyphae formation; one-way ANOVA p-value is 4.67 × 10−12. Different letters indicate significant differences and similar letters indicate no difference according to Tukey’s single-step multiple comparison procedure. Each LCO concentration was analyzed in three independent wells. In the box plots (b, c), the bars represent the minimum value, the first quartile, the median, the third quartile, and the maximum value such that 25% of the data are in each section. Source data are provided as a Source Data file.