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. 2020 Jul 29;11:1752. doi: 10.3389/fmicb.2020.01752

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Copyright © 2020 Bañuelos-Vazquez, Cazares, Rodríguez, Cervantes-De la Luz, Sánchez-López, Castellani, Tejerizo and Brom.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Flow cytometry analysis of bacteria recovered from the root surface and nodules of plants infected with CFNX182-1. Plants were inoculated with strain CFNX182-1, which carries a RFP marker on the chromosome and a GFP marker on the pSym. Bacteria were recovered at 20 dpi from: (A) the medium, (B) the root surface and (C) nodules. Populations corresponding to donors (D) show red plus green fluorescence, putative endophytic recipients (PE) have no fluorescence, transconjugants of endophytic recipients (T) carry GFP and show green fluorescence, and pSym-cured derivatives (C) with RFP show red fluorescence.