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. 2020 Jul 29;11:1752. doi: 10.3389/fmicb.2020.01752

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Copyright © 2020 Bañuelos-Vazquez, Cazares, Rodríguez, Cervantes-De la Luz, Sánchez-López, Castellani, Tejerizo and Brom.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Visualization of roots, infection threads and nodules of P. vulgaris plants infected with CFNX182-1 and TER strains. Plants were inoculated with the donor strain CFNX182-1 (A–C), and with transconjugant strains TER31 (D–F), TER38 (G–I) and TER49 (J–L). Samples of the root surface (A,D,G,J), infection thread (B,E,H,K), and nodules (C,F,I,L) were analyzed 21 dpi by confocal microscopy. CFNX182-1 is yellow (red plus green fluorescence) and transconjugants of endophytic recipients (TER) carry green fluorescence. Arrowheads indicate the localization of transconjugants (C).