R. etli |
|
|
|
CFNX182 |
Derivative of CFN42, cured of pRet42a |
Nal |
Brom et al., 1992 |
CFNX182-1 |
Derivative of CFNX182 with RFP in the chromosome and GFP and SpR in pRet42d |
Nal, Gm, Sp, RFP and GFP |
This work |
TER |
|
|
|
TER22 |
Strain isolated from nodules infected with CFNX182-1 |
Sp, GFP |
This work |
TER23 |
Strain isolated from nodules infected with CFNX182-1 |
Sp, GFP |
This work |
TER31 |
Strain isolated from nodules infected with CFNX182-1 |
Sp, GFP |
This work |
TER33 |
Strain isolated from nodules infected with CFNX182-1 |
Sp, GFP |
This work |
TER38 |
Strain isolated from nodules infected with CFNX182-1 |
Sp, GFP |
This work |
TER39 |
Strain isolated from nodules infected with CFNX182-1 |
Sp, GFP |
This work |
TER40 |
Strain isolated from nodules infected with CFNX182-1 |
Sp, GFP |
This work |
TER49 |
Strain isolated from nodules infected with CFNX182-1 |
Sp, GFP |
This work |
TER55 |
Strain isolated from nodules infected with CFNX182-1 |
Sp, GFP |
This work |
E. coli |
|
|
|
DH5α |
supE44 ΔlacU169 (Φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 |
Nal |
Sambrok et al., 1989 |
S17-1 |
E. coli 294:[RP4-2 (Tc:Mu) (Km:Tn7)] thi pro hsdR hsdM ΔrecA |
Km |
Simon, 1984 |
Plasmids |
|
|
|
pK18mob |
High copy number cloning vector |
Km |
Schäfer et al., 1994 |
pK18mob-sacB |
Cloning vector, mobilizable |
Km |
Schäfer et al., 1994 |
pCRTM2.1-TOPOTM
|
Cloning vector |
Km, Amp |
InvitrogenTM
|
pGX33 |
Derivative of pCRTM2.1-TOPOTM with a 579 pb fragment from pRet42d |
Km, Amp |
This work |
pGX77 |
Derivative of pK18mob-SacB harboring a pRet42d-fragment from pGX33 (cloned as a PstI and HindIII fragment) |
Km |
This work |
pGX537 |
Vector that carries a 4.1 Kbp-NotI cassette (promoter NptII -gfpmut3∗- ΩSp) |
Km, Sp |
Torres Tejerizo et al., 2015 |
pGX542 |
Derivative of pGX77 harboring the NotI-cassette from pGX537. |
Km, Sp |
This work |