TABLE 1.
Strains and plasmids used in this work.
| Strains and plasmids | Relevant characteristics | Features | References |
| R. etli | |||
| CFNX182 | Derivative of CFN42, cured of pRet42a | Nal | Brom et al., 1992 |
| CFNX182-1 | Derivative of CFNX182 with RFP in the chromosome and GFP and SpR in pRet42d | Nal, Gm, Sp, RFP and GFP | This work |
| TER | |||
| TER22 | Strain isolated from nodules infected with CFNX182-1 | Sp, GFP | This work |
| TER23 | Strain isolated from nodules infected with CFNX182-1 | Sp, GFP | This work |
| TER31 | Strain isolated from nodules infected with CFNX182-1 | Sp, GFP | This work |
| TER33 | Strain isolated from nodules infected with CFNX182-1 | Sp, GFP | This work |
| TER38 | Strain isolated from nodules infected with CFNX182-1 | Sp, GFP | This work |
| TER39 | Strain isolated from nodules infected with CFNX182-1 | Sp, GFP | This work |
| TER40 | Strain isolated from nodules infected with CFNX182-1 | Sp, GFP | This work |
| TER49 | Strain isolated from nodules infected with CFNX182-1 | Sp, GFP | This work |
| TER55 | Strain isolated from nodules infected with CFNX182-1 | Sp, GFP | This work |
| E. coli | |||
| DH5α | supE44 ΔlacU169 (Φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Nal | Sambrok et al., 1989 |
| S17-1 | E. coli 294:[RP4-2 (Tc:Mu) (Km:Tn7)] thi pro hsdR hsdM ΔrecA | Km | Simon, 1984 |
| Plasmids | |||
| pK18mob | High copy number cloning vector | Km | Schäfer et al., 1994 |
| pK18mob-sacB | Cloning vector, mobilizable | Km | Schäfer et al., 1994 |
| pCRTM2.1-TOPOTM | Cloning vector | Km, Amp | InvitrogenTM |
| pGX33 | Derivative of pCRTM2.1-TOPOTM with a 579 pb fragment from pRet42d | Km, Amp | This work |
| pGX77 | Derivative of pK18mob-SacB harboring a pRet42d-fragment from pGX33 (cloned as a PstI and HindIII fragment) | Km | This work |
| pGX537 | Vector that carries a 4.1 Kbp-NotI cassette (promoter NptII -gfpmut3∗- ΩSp) | Km, Sp | Torres Tejerizo et al., 2015 |
| pGX542 | Derivative of pGX77 harboring the NotI-cassette from pGX537. | Km, Sp | This work |