TABLE 2.

Characteristics of putative transconjugants from endophytic recipients isolated from nodules.

Name	Genusa	Class	Coverage –identityb	nodD1c	nodAc	fixNdc	nifHc	GFPc	Nodulationd
TER22	Brevundimonas sp.	Alphaproteobacteria	100–99.88%	+	+	+	+	+	+
TER23	Pseudomonas sp.	Gammaproteobacteria	100–100%	+	+	+	+	+	−
TER31	Stenotrophomonas sp.	Gammaproteobacteria	100–94.16%	+	+	+	+	+	+
TER33	Bacillus sp.	Firmicutes-Bacilli	100–99.88	+	+	+	+	+	−
TER38	Achromobacter sp.	Betaproteobacteria	100–99.25%	+	+	+	+	+	+
TER39	Ochrobactrum sp.	Alphaproteobacteria	100–100%	+	+	+	+	+	−
TER40	Mesorhizobium sp.	Alphaproteobacteria	99–98.75%	+	+	+	+	+	−
TER49	Phyllobacterium sp.	Alphaproteobacteria	100–98.64%	+	+	+	+	+	+
TER55	Achromobacter sp.	Betaproteobacteria	99–98.61%	+	+	+	+	+	−

^aGenera were identified by sequencing PCR products obtained with 16S primers, using DNA of each strain as template. ^bThe coverage and identity were determined by BLASTn of the internal fragments of the 16s RNA. ^cPresence of R. etli CFNX182-1 pSym sequence was determined by the obtention of PCR products of the different genes, using DNA of each strain as template. ^dNodulation ability was determined by inoculating bean seedlings with cultures of each purified strain, and determining the presence of nodules at 20 dpi.