TABLE 1.
List of strains and plasmids used in this study.
| Strains | Genotype | Source |
| BY4741 | MATa his3 leu2 met15 ura3 | Euroscarf |
| NDY257 | BY4741 rtn1::kanMX4 yop1::kanMX4 rtn2::kanMX4 | Voeltz et al., 2006 |
| SEY6210.1 | MATa leu2−3,112 ura3−52 his3−Δ200 trp1−Δ901 lys2−801 suc2−Δ9 | Manford et al., 2012 |
| ANDY214 | SEY6210.1 tcb1Δ::KANMX6 tcb2Δ::KANMX6 tcb3Δ::HISMX6 | Manford et al., 2012 |
| Sey1-TAP | S288C; ATCC 201388; MAT a his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 SEY1-TAP::HIS3MX6 | GE Dharmacon |
| Yop1-TAP | S288C; ATCC 201388; MAT a his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 YOP1-TAP::HIS3MX6 | GE Dharmacon |
| Vph1-mCherry | BY4741 VPH1-mCherry::LEU2 | This study |
| rtn1Δ rtn2Δ yop1Δ Vph1-mCherry | BY4741 rtn1::kanMX4 yop1::kanMX4 rtn2::kanMX4 VPH1-mCherry::LEU2 | This study |
| Erg6-RFP | MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 ERG6::mRFP kanMX6 | Huh et al., 2003 |
| Rtn1-GFP | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Rtn1-GFP:: His3MX | Huh et al., 2003 |
| Plasmids | Description | |
| pRS426-G20 | TEF2 promoter, GFP-Spo2051–91 (2 μ, URA3) | Nakanishi et al., 2004 |
| pGPD416-C1δ-GFP | GPD promoter, C1δ-GFP (CEN, URA3) | Ganesan et al., 2018 |
| pESC LEU2 | GAL1/10 promoter (2 μ, LEU2) | Agilent |
| YEplac181-GAL1/10-DGK1 | DGK1 under control of GAL1/10 promoter in YEplac181 (2 μ, LEU2) | Han et al., 2008 |
| YEplac181-GAL1/10-DGK1[D177A] | DGK1[D177A] under control of GAL1/10 promoter in YEplac181 (2 μ, LEU2) | Han et al., 2008 |