Figure 1.

Immunostaining for Th reveals the striped distribution of the Th+ Purkinje cells (PCs) in the cerebellar cortex of the posterior vermis and parafloccular/floccular complex. (A) Immunostaining for Th reveals physiological Th expression in the cerebellum. Expression in lobules IX and X are shown in the coronal section. (B) Robust immunoreactivity for Th is identified in a subset of Purkinje cells that are arranged in parasagittal stripes. Cell bodies and dendrites of these PCs were labeled. ml, molecular layer; pc, Purkinje cell layer; gl, granule cell layer. (C) High magnification of Th+ PCs demonstrates Th+ puncta (arrowhead) that are in close apposition to PCs. Purkinje cell bodies and apical dendrites (Arrow) again show intense immunoreactivity for Th. ml, molecular layer; pc, Purkinje cell layer; gl, granule cell layer. (D) Serial section alignment analysis for the entire vermal cortex to visualize stripes of Th+ PCs. The result demonstrates an unfolded flat map of the vermal cortex reconstructed from the serial cerebellar sections immunostained for Th (see “Materials and Methods” section). (E) Schematic representation of the striped distribution of the Th+ vermal PCs. Drawn from the analysis in panel (D). Red dotted lines indicate the approximate location of the images in panel (H). (F) Th-immunoreactive PCs identified in the parafloccular/floccular (PFL/FL) complex. (G) Schematic representation of the distribution of the Th+ parafloccular/floccular PCs. (H) Double immunostaining for Th and Aldoc of cerebellar sections. Colabeling of Th (magenta) and Aldoc (green) is revealed in a subset of Aldoc+ Purkinje cells. Images of lobules IXc or VII, at levels indicated in (B), are shown. Below each panel is magenta and green bars that represent striped expression patterns of Th and Aldoc, respectively. Inset shows a higher magnification image of PCs, at an area marked with a white rectangle, that is colabeled with Th and Aldoc.