Figure 5.

Conditional knockout of Th in all Purkinje cells results in altered behavioral flexibility and response inhibition, but not instrumental learning, or working memory. (A) Schematic of delayed alternation test for working memory, adapted from Beas et al. (2017). (B) Performance between Pcp2-Cre;Th^lox/lox mice (Green, n = 8) and littermate controls (Black, n = 8) in delayed alternation with a 2-s delay. No difference observed between groups or the effect of interaction between group and time. Error bars are SEM. (C) Schematic of reversal of delayed alternation test for behavioral flexibility, adapted from Beas et al. (2017). (D) Performance of Pcp2-Cre;Th^lox/lox mice (Green, n = 8) relative to littermate controls (Black, n = 8) in performance of delayed alternation reversal with 8-s delay. There was an effect of genotype group, F_(1,13) = 5.96, *p < 0.03 and training F_(3,39) = 6.68, *p < 0.001, but not for an effect between group × training F_(3,39) = 1.23, p = 0.313. Two-way rmANOVA. Error bars are SEM. (E) Schematic of differential reinforcement of low rate responses test for impulsive pressing, adapted from Nautiyal et al. (2015) and Locke et al. (2018). (F) Burst pressing performance (presses <3 s after last rewarded press, ITI = intertrial interval) of Pcp2-Cre;Th^lox/lox mice (Green, n = 6) relative to littermate controls (black, n = 7) on DRL task over 4 days of training. There was an effect of group, F_(1,11) = 6.59, *p < 0.03. There was not an effect of training or the effect of interaction between group and training. Two-way rmANOVA. Error bars are SEM.