Figure 10.
Summary of berbamine analog protection and compound characteristics. Compound name, structure, molecular weight, protection class, likely mechanism of protection, ED50, and OPCs against neomycin and gentamicin for all 16 berbamine analogs. The general protection classes were defined based on thresholds of protection levels by percent. Robust = 60% increase in hair cell protection compared to aminoglycoside group; Strong = over 40% increase in hair cell protection compared to aminoglycoside group; Modest = over 30% increase in hair cell protection compared to aminoglycoside group; No protection = less than 30% of hair cell protection compared to aminoglycoside group. The likely mechanism was determined by the GTTR experiments in Figure 6 and the time course experiments shown in Figure 8 and Supplementary Figure S1.