Figure 5.

Hair cell protection persists after 24 h. Zebrafish were pretreated with the OPC of analog for 1 h followed by 200 μM gentamicin treatment for 30 min. Fish were allowed to recover for 24 h before the assessment. All analogs tested significantly prevented hair cell death after 24 h suggesting true protection rather than a delay in cell death onset. Hair cells were assessed via DASPEI. Data are presented normalized to vehicle controls. Data were analyzed by one-way ANOVA, ****p < 0.0001, ***p < 0.001, F_(4,32) = 12.98, N = 5–10, bars are ± SEM.