Table 2:
Antibodies used in this study
| Fluorochrome labelled antibodies | Clone | Source | Catalog number |
|---|---|---|---|
| Siglec F-eFluor660 | 1RNM44N | Thermo Fisher Scientific | 50-702-82 |
| SiglecF-BV421 | E50–2440 | BD Biosciences | 562681 |
| CD11c-APC-eFluor780 | N418 | Thermo Fisher Scientific | 47-0114-82 |
| F4/80-eFluor450 | BM8 | BioLegend | 123132 |
| CD11b-PE-TexasRed | M1/70.15 | Thermo Fisher Scientific | RM2817 |
| I-A/I-E (MHC-ll)-PE-Cy7 | M5/114.15.2 | Thermo Fisher Scientific | 25-5321-82 |
| I-A/I-E (MHC-ll)-eFluor450 | M5/114.15.2 | Thermo Fisher Scientific | 48-5321-82 |
| CD103-PerCP-Cy5.5 | M290 | BD Biosciences | 563637 |
| CD3-Alexa Fluor 488 | 145-2C11 | Thermo Fisher Scientific | 53-0031-82 |
| CD45R(B220)-Alexa Fluor 488 | RA3-6B2 | Thermo Fisher Scientific | 53-045-82 |
| CCR3-PE | 83101 | R&D systems | FAB729P |
| Gr1-(Ly6G/Ly6C)-APC | RB6-8C5 | BioLegend | 108412 |
| Lin-FITC Ms | - | BioLegend | 78022 |
| CD25-PE | PC61.5 | Thermo Fisher Scientific | 12-0251-81 |
| KLRG1-PE-Cy7 | 2F1/KLRG1 | BioLegend | 138415 |
| Sca1-BV421 | D7 | BioLegend | 108128 |
| CD45-APC | 30-F11 | BioLegend | 103112 |
| cKit-Alexa fluor700 | ACK2 | Thermo Fisher Scientific | 56-1172-82 |
| CD90.2 (Thy1.2)-APC-Cy7 | 53–2.1 | Thermo Fisher Scientific | 47-0902-82 |
| CD40-FITC | 3/23 | BioLegend | 124608 |
| CD40-APC | 1C10 | Thermo Fisher Scientific | 17-0401-82 |
| CD80-PE-Cy5 | 1610A1 | Thermo Fisher Scientific | 15-0801-82 |
| CD86-PE | PO3 | Thermo Fisher Scientific | MA5-16921 |
| CD209a (DC-SIGN)-eFluor660 | MMD3 | Thermo Fisher Scientific | 50-2094-80 |
| Dectin1-PE | RH1 | BioLegend | 144303 |
| Dectin2-Alexa Fluor 647 | D2.11E4 | Biorad | MCA2415A647T |
| Glut1-PE | Polyclonal | Novus Biologicals | NB110-39113PE |
| Yellow fluorescent stain | - | Thermo Fisher Scientific | L34967 |