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. 2020 Aug 4;10:13138. doi: 10.1038/s41598-020-70068-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of Zonisamide (ZNS) on the number of ChAT-positive motor neurons in the anterior horn (AH) region. (A–C) Representative confocal images of the anterior horn (AH) region in the spinal cord at the C5-6 disc level of sham-operated rat and CSM rat with [ZNS( +)] or without [ZNS(-)] ZNS administration. Sections were immunostained with an antibody against choline acetyltransferase (ChAT, red). ChAT-positive cells are indicated by white arrowheads. Bar = 50 μm. (D) The number of ChAT-positive cells in the AH region in sham-operated, ZNS(-) and ZNS( +) rats. Mean and SD are indicated (n = 6 anterior horns of 3 rats for each group). * p < 0.05, and ** p < 0.01 by Kruskal–Wallis test followed by Dunn-Bonferroni HSD.