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. 2020 Aug 4;10:13079. doi: 10.1038/s41598-020-70157-0

Figure 2.

Figure 2

miR-4634 inhibits the proliferation and migration of A549 cells. (A, B) Cells were infected by LV-miR-4634 and transfected with miR-4634 mimics. The expression of miR-4634 was determined by qRT-PCR. All data were analyzed by the t-test. (C) Stable A549 cells, as indicated, were seeded in 96-well plates and then detected by CCK-8 at 0 h, 24 h, 48 h, 72 h, and 96 h. The line chart was performed using GraphPad Prism V5.0 (https://www.graphpad.com/). (D) Stable A549 cells were seeded in 6-well plates at a density of 1,000 cells per well. The same treatment (DMSO or 5 nM RAD001) was repeated every 3 days until day 10. The colonies in each well were counted. (E) Migration of A549 cells was observed at 0 h and 24 h following wounding by a pipette tip. Original magnification: 100 × . (F) Cellular migration ability was analyzed by transwell assay after 16 h treatment. Original magnification: × 200. Bars are means of three replicate determinations plus standard deviations. All data were analyzed by the one-way ANOVA. NS, non-significant, * P < 0.05, ** P < 0.01, ***P < 0.001.