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. 2020 Aug 4;10:13141. doi: 10.1038/s41598-020-69969-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of promoters of cap-dependent translation initiation, and synaptic plasticity regulators in prefrontal cortex following 3 days active work (AW, collected at zeitgeber time, ZT0) and rest work (RW, collected at ZT12). (A,B) m7GTP pull-down analysis. (C) Representative immunoblots for (A,B). Blots normalized to total eIF4E in the corresponding immunoblot. Phospho-protein normalized to total protein in the corresponding immunoblot. (D,E) Western blot analysis. (F) Representative immunoblots for (D,E). Full-length blots/gels are presented in Supplementary Figure S2. Blots normalized to GAPDH in the corresponding immunoblots. Phospho-protein normalized to total protein in the corresponding immunoblot. Quantification of immunoblots are expressed as percentage change relative to time-matched undisturbed control (AWC collected at ZT0, RWC collected at ZT12; normalized to 100%). Plots show mean ± SEM, with scatter plot overlaid. N = 6–9/group. *p < 0.05, **p < 0.01, compared to time-matched control.