Figure 1.

Toxoplasma-driven IFN-γ secretion by non-CD4 immune cells following oral infection with brain cysts or intravenous (i.v.) infection with tachyzoites. (a,b) Percent of IFN-γ⁺ cells amongst total viable CD3⁺CD4⁺, CD3⁺CD8⁺, CD3⁺CD4^–CD8^– (DN) T cells and CD3^–NKp46⁺ cells in the spleen 1 day (a) or 5 days (b) after B6 mice were inoculated orally with 10, 40 or 100 T. gondii ME49 brain cysts. (c) Percent of IFN-γ⁺ cells amongst total viable CD3⁺CD4⁺, CD3⁺CD8⁺, CD3⁺CD4^–CD8^– (DN) T cells and CD3^–NKp46⁺ cells in the spleen 2, 24, 48 or 72 h after B6 mice were injected i.v. with 10⁷ T. gondii ME49 tachyzoites. (d) Percent of IFN-γ⁺ cells amongst total viable CD3⁺CD4⁺, CD3⁺CD8⁺, CD3⁺CD4^–CD8^– (DN) T cells and CD3^–NKp46⁺ cells in the spleen 24 h after B6 mice were injected i.p. with 10⁷ T. gondii ME49 tachyzoites. (e,f) Percent of IFN-γ⁺ cells amongst total viable CD3⁺CD4⁺, CD3⁺CD8⁺, CD3⁺CD4^–CD8^– (DN) T cells and CD3^–NKp46⁺ cells in mesenteric lymph nodes (MLN) 1 day (e) or 5 days (f) after B6 mice were inoculated orally with 10, 40 or 100 T. gondii ME49 brain cysts. (g) Percent of IFN-γ⁺ cells amongst total viable CD3⁺CD4⁺, CD3⁺CD8⁺, CD3⁺CD4^–CD8^– (DN) T cells and CD3^–NKp46⁺ cells in MLN 2, 24, 48 or 72 h after B6 mice were injected i.v. with 10⁷ T. gondii ME49 tachyzoites. (h) Percent of IFN-γ⁺ cells amongst total viable CD3⁺CD4⁺, CD3⁺CD8⁺, CD3⁺CD4^–CD8^– (DN) T cells and CD3^–NKp46⁺ cells in MLN 24 h after B6 mice were injected i.p. with 10⁷ T. gondii ME49 tachyzoites. (i–k) Serum concentrations of IL-6 (i), TNFα (j) and IL-10 (k) at 2–72 h after B6 mice were injected i.v. with 10⁷ T. gondii ME49 tachyzoites. Results are presented as pooled data means ± SEM from at least two pooled independent experiments (n = 5–10 mice per group). See also Figure S1.