Fig. 4.

Core metabolic pathways and their proteomic presence within Euglena gracilis. (A) TCA cycle showing the conventional pathway rendered nonfunctional by absence of key protein subunits along with alpha-proteobacterial shunt and aminobutyric (GABA) shunt. Components involved only in the glyoxylate cycle are transparent. (B) Glyoxylate cycle, highlighting associated enzymes from TCA cycle. Blue for proteomically confirmed sequences, light blue for sequences identified in transcriptome, and gray for absent sequences. Duplicated sequences are available in supplementary table 7, Supplementary Material online.