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. 2020 Jun 17;21(8):e49823. doi: 10.15252/embr.201949823

Figure EV1. Gating strategies for the assessment of B‐ and T‐cell populations in WT and Shld2 −/− mice.

Figure EV1

  • A
    Gating strategies used to quantitate Hardy fractions A‐F of progenitor B‐cells in bone marrows of WT and Shld2 −/− mice.
  • B
    Gating strategies used to quantitate immature, mature, T1, T2, MZ, and FO B‐cell populations in the spleen of WT and Shld2 −/− mice.
  • C
    Gating strategies used to quantitate B1, B2, B1a, and B1b populations in the peritoneal cavity of WT and Shld2 −/− mice.
  • D
    Gating strategies used to quantitate various thymocyte populations in WT and Shld2 −/− mice.
  • E
    Insertion–deletion (indel) penetrance was measured by TIDE sequencing for the lentiCRISPRv2 constructs expressing the indicated sgRNAs targeting the 53bp1, Shld1, Shld2, Shld3, and Lig4 genes.
  • F
    Baseline GFP frequency of bulk gene‐edited A70.2 cells prior to imatinib stimulation (Fig 1F).