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. 2020 Jun 17;21(8):e48462. doi: 10.15252/embr.201948462

Figure 2. CTGF stabilizes Lrp4 on the plasma membrane of C2C12 myotube and the embryonic muscle.

Figure 2

  • A
    Representative immunoblotting of Lrp4, MuSK, transferrin receptor (TfR), CTGF, and β‐actin in whole‐cell lysates and the plasma membrane fraction of C2C12 myotubes treated with 1 pM (1 ng/ml) agrin and/or 250 pM (100 ng/ml) CTGF for 24 h. CTGF increases Lrp4 on the plasma membrane in the presence of agrin.
  • B
    C2C12 myotubes were treated with 1 pM agrin and/or 250 pM CTGF for 6 h. Lrp4 was immunoprecipitated (IP) with an anti‐Lrp4 antibody, and phosphorylated Lrp4 was immunoblotted with an anti‐phosphotyrosine (p‐Tyr) antibody.
  • C
    Representative immunoblotting of Lrp4, MuSK, CTGF, and TfR in whole tissue lysates and the plasma membrane fraction of lower limb muscles at E18.5 of wild‐type (WT) and Ctgf −/− mice.
  • D
    Representative immunoblotting of Y755‐phosphorylated MuSK, total MuSK, and β‐actin in the diaphragm at E18.5 of wild‐type (WT), Ctgf −/−, and Ctgf +/− mice.
Data information: Mean and SD (n = 3 independent experiments) are indicated. P‐value < 0.05 by one‐way ANOVA. P < 0.05 by post hoc Tukey test is indicated by a single letter representing each group.Source data are available online for this figure.